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B-hBCMA/hGPRC5D MC38
Common name
B-hBCMA/hGPRC5D MC38 Catalog number    322252
Aliases

BCM, BCMA, CD269, TNFRSF13A; /

Disease  Colon carcinoma
Organism
Mouse 
Strain  C57BL/6
Tissue types Colon Tissue  Colon
Protein expression analysis 

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BCMA and GPRC5D expression analysis in B-hBCMA/hGPRC5D MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hBCMA/hGPRC5D MC38 cultures were stained with species-specific anti-BCMA antibody (in house) and anti-GPRC5D antibody. Human BCMA and GPRC5D were detected on the surface of B-hBCMA/hGPRC5D MC38 cells. The 3-D03 clone of B-hBCMA/hGPRC5D MC38 cells was used for in vivo tumor growth assays.

Tumor growth curve & body weight changes

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Subcutaneous homograft tumor growth of B-hBCMA/hGPRC5D MC38 cells. B-hBCMA/hGPRC5D MC38 cells (5x105; 1x106; 5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-10-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hBCMA/hGPRC5D MC38 cells were able to form tumors in vivo and can be used for efficacy studies.