top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hGAS6 MC38
Common name
B-hGAS6 MC38 Catalog number    321847
Aliases

AXSF; AXLLG

Disease  Colon carcinoma
Organism
Mouse 
Strain  C57BL/6
Tissue types Colon Tissue  Colon
Description 

The human GAS6 CDS was inserted into mouse Gas6 gene, so that the mouse Gas6 was not expressed anymore, human GAS6 was expressed in B-GAS6 MC38 cells. 

Application

B-hGAS6 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hGAS6 MC38 cells. The exogenous promoter and human GAS6 coding sequence was inserted to replace part of murine exon 1 and all of exon 2. The insertion disrupts the endogenous murine Gas6 gene, resulting in a non-functional transcript.

Protein expression analysis 

from clipboard


GAS6 expression analysis in B-hGAS6 MC38 cells by ELISA. Supernatant from wild-type MC38 and B-hGAS6 MC38 cultures were stained with species-specific anti-GAS6 antibody. Human GAS6 was detected in the supernatant of B-hGAS6 MC38 cells but not wild-type MC38 cells. The 8-H12 clone of B-hGAS6 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

from clipboard

Subcutaneous homograft tumor growth of B-hGAS6 MC38 cells. B-hGAS6 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hGAS6 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.


Tumor growth curve of individual mice


from clipboard

B-hGAS6 MC38 tumor growth of individual mice. B-hGAS6 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 6-week-old, n=6). As shown in panel, B-hGAS6 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.


Protein expression analysis of tumor cells


from clipboard

B-hGAS6 MC38 cells were subcutaneously transplanted into C57BL/6 mice (n=6). At the end of the experiment, tumors were harvested and assessed for GAS6 expression by ELISA. As shown, human GAS6 was highly expressed by tumor cells. Therefore, B-hGAS6 MC38 cells can be used for in vivo efficacy studies.