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B-Bcrp KO rats
Strain Name SD-Abcg2tm1Bcgen/Bcgen   Common Name  B-Bcrp KO rats
Background SD Catalog number 113096
Aliases 

ABC15, ABCP, BCRP, BCRP1, BMDP, 

CD338, CDw338, CDw388, EST157481, 

GOUT1, MRX, MXR, MXR-1, MXR1, UAQTL1

NCBI Gene ID
9429
Description

  • BCRP consists of 655 amino acids and possesses six transmembrane helices and one ATP­ binding site. Because BCRP is a half­-transporter, current evidence suggests that homodimerization or multimerization is required for transporter activity as illustrated. 
  • The exons 2~16 of rat Bcrp gene were knocked out in B-Bcrp KO rats, resulting in a disruption of the Bcrp gene.
  • Bcrp mRNA and protein were detectable only in wild-type SD rats, but not in B-Bcrp KO rats. 
Suggestion:
  • Applicable for investigating ATP-powered transport proteins in physiological drug distribution.

Targeting strategy

Gene targeting strategy for B-Bcrp KO rats. The exons 2~16 of mouse Bcrp gene were knocked out in B-Bcrp KO rats, resulting in a disruption of the Bcrp gene.

mRNA expression analysis in B-Bcrp KO rats

from clipboard

Bcrp mRNA expression in wild-type SD rats and B-Bcrp KO rats by RT-PCR. Kidney, brain and liver RNA were isolated from wild-type SD rats (+/+) and B-Bcrp KO rats (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with rat Bcrp primers. Rat Bcrp mRNA was detectable only in wild-type SD rats, but not in B-Bcrp KO rats.

Protein expression analysis

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Western blot analysis of BCRP protein expression in B-Bcrp KO rats. Various tissue lysates were collected from wild-type SD rats (+/+) and B-Bcrp KO rats (-/-), and then analyzed by western blot with anti-BCRP antibody (abcam, ab207732). 40 μg total proteins were loaded for western blotting analysis. BCRP was not detectable in B-Bcrp KO rats.