B-Ldlr KO mice_Biocytogen Pharmaceuticals (Beijing) Co., Ltd._Biocytogen, drug development, antibody discovery

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B-Ldlr KO mice

Strain Name	C57BL/6JNifdc-Ldlr^tm1Bcgen/Bcgen	Common Name	B-Ldlr KO mice
Background	C57BL/6JNifdc	Catalog number	113270
Aliases	FH, FHC, FHCL1, LDLCQ2
NCBI Gene ID	3949

Description

LDLR, or Low-Density Lipoprotein Receptor, is a critical cell surface receptor involved in cholesterol metabolism. It facilitates the uptake of LDL particles, which contain high levels of cholesterol, by binding to apolipoproteins B-100 and E.
Expression the LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.
Gene editing strategy: the exons 4-18 of mouse Ldlr gene were knocked out in B-Ldlr KO mice, resulting in a disruption of the Ldlr gene.
Application: This product is used for pharmacodynamics evaluation of metabolic diseases.

Targeting strategy

Gene targeting strategy for B-Ldlr KO mice. The exons 4-18 of mouse Ldlr gene were knocked out in B-Ldlr KO mice, resulting in a disruption of the Ldlr gene.

mRNA expression analysis in humanized B-Ldlr KO mice

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Species specific analysis of Ldlr gene expression in wild-type C57BL/6JNifdc mice and homozygous B-Ldlr KO mice by RT-PCR. Liver was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-). Mouse Ldlr mRNA was detectable only in wild-type C57BL/6JNifdc mice, but not in homozygous B-Ldlr KO mice.

Protein expression analysis

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Western blot analysis of LDLR protein expression in homozygous B-Ldlr KO mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-), and then analyzed by western blot with anti-mouse LDLR antibody (R&D, AF2255). 40 μg total proteins were loaded for western blotting analysis. LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.