| Strain Name | C57BL/6JNifdc-Ldlrtm1Bcgen/Bcgen | Common Name | B-Ldlr KO mice |
| Background | C57BL/6JNifdc | Catalog number | 113270 |
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Aliases |
FH, FHC, FHCL1, LDLCQ2 |
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NCBI Gene ID |
3949 | ||
- LDLR, or Low-Density Lipoprotein Receptor, is a critical cell surface receptor involved in cholesterol metabolism. It facilitates the uptake of LDL particles, which contain high levels of cholesterol, by binding to apolipoproteins B-100 and E.
- Expression the LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.
- Gene editing strategy: the exons 4-18 of mouse Ldlr gene were knocked out in B-Ldlr KO mice, resulting in a disruption of the Ldlr gene.
- Application: This product is used for pharmacodynamics evaluation of metabolic diseases.
Western blot analysis of LDLR protein expression in homozygous B-Ldlr KO mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-Ldlr KO mice (-/-), and then analyzed by western blot with anti-mouse LDLR antibody (R&D, AF2255). 40 μg total proteins were loaded for western blotting analysis. LDLR was only detected in wild-type mice, but not in homozygous B-Ldlr KO mice.
Analysis of lipid metabolism after Ldlr knockout in B-Ldlr KO mice
Lipid metabolism analysis in B-Ldlr KO mice. Serum concentrations of TG, TC, LDL-C, and HDL-C were analyzed in 4-hour-fasted B-Ldlr KO mice (-/-) and wild-type C57BL/6JNifdc mice (+/+) (6 weeks old). The levels of LDL-C, TC, and TG in B-Ldlr KO mice were significantly higher than those in wild-type C57BL/6JNifdc mice.
TG, triglycerides; TC, total cholesterol; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; F, female; M, male.
Values are expressed as mean ± SEM. Significance was determined by a two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
