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B-Mdr1 KO rats
Strain Name SD-Abcb1atm1Bcgen Abcb1btm1Bcgen/Bcgen   Common Name  B-Mdr1 KO rats
Background SD Catalog number 113090
Aliases 

ABC20, CD243, CLCS, ENPAT, GP170, MDR1, P-GP, PGY1, p-170

NCBI Gene ID
5243
Description

  • Mdr1 is predominantly expressed in the intestine, blood-brain barrier, blood-testis barrier, and adrenal glands, pregnant uterus, and ovaries. These P-glycoproteins actively pump drugs out of cells, contributing to multidrug resistance in tumors. Their absence disrupts blood-brain barrier protection and increases systemic drug exposure. 
  • The genome sequences between rat Mdr1a gene and Mdr1b gene were depleted in B-Mdr1 KO rats. As a result, rat MDR1 protein will not be expressed anymore.
  • Mdr1 mRNA and protein were detectable only in wild-type SD rats, but not in B-Mdr1 KO rats. 
Suggestion:
  • This model is valuable for studying CNS drug delivery, neurotoxicology, oral bioavailability, and mechanisms of multidrug resistance.

Targeting strategy

Gene targeting strategy for B-Mdr1 KO rats. The genome sequences between rat Mdr1a gene and Mdr1b gene were depleted in B-Mdr1 KO rats. As a result, rat MDR1 protein will not be expressed anymore.

mRNA expression analysis in B-Mdr1 KO rats

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Mdr1 mRNA expression in wild-type SD rats and B-Mdr1 KO rats by RT-PCR. Liver RNA were isolated from wild-type SD rats (+/+) and B-Mdr1 KO rats (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with rat Mdr1 primers. Rat Mdr1 mRNA was detectable only in wild-type SD rats, but not in B-Mdr1 KO rats.

Protein expression analysis

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Western blot analysis of Mdr1 protein expression in B-Mdr1 KO rats. Various tissue lysates were collected from wild-type SD rats (+/+) and B-Mdr1 KO rats (-/-), and then analyzed by western blot with anti-Mdr1 antibody (abcam, ab170904). 40 μg total proteins were loaded for western blotting analysis. Mdr1 was not detectable in B-Mdr1 KO rats.