Mouse Immunodeficiency System CDX Model

Xenotransplantation of human-derived tumor cell lines (CDX) refers to the tumor model established by inoculating human tumor cells into immunodeficient mice (such as severely immunodeficient B-NDG mice) after in vitro subculture. The commonly used inoculation sites are subcutaneous, orthotopic or intravenous. This tumor model has the advantages of good modeling stability and high success rate, and is widely used in tumor cell proliferation research and in the screening of anti-tumor drugs in vivo.

Using our self-developed immunodeficient B-NDG mice, Biocytogen establishes a variety of solid tumor models for anti-tumor drug screening, including lung cancer, rectal adenocarcinoma, colon cancer, breast cancer, pancreatic cancer, bladder cancer, liver cancer and a variety of hematological tumor models such as acute lymphoid leukemia and acute myeloid leukemia. Using these models, we  provide high-quality pharmacodynamic (PD) services.

Xenograft model ^[1]

Xenograft Model ^[2]

Case 1: Melanoma Model

Human melanoma A2058 cells were inoculated subcutaneously in B-NDG mice to successfully establish a melanoma CDX model.

A. Tumor growth curve, B. Body weight change of tumor-bearing mice

Case 2: Human B-cell lymphoma model

The B cell lymphoma model was successfully established by injecting Raji cells into B-NDG, NOD-scid and BALB/C nude mice via the tail vein.

(A) Kaplan-Merier survival curves, (B) relative body weight change, (C) q-PCR quantification of peripheral blood human cell ratios in mice, (D) liver tumors, (E) immunohistochemical staining of liver and spleen

Case 3: Hepatoma Model

A. Tumor growth curve, B. Body weight change of tumor-bearing mice

Case 4: Colon Cancer Model

Human colorectal adenocarcinoma HT-29 cells were inoculated subcutaneously in B-NDG mice to successfully establish a colorectal adenocarcinoma model.

A. Tumor growth curve, B. Body weight change of tumor-bearing mice

Available CDX models

CDX Models Using Luciferase-Expressing Cell Lines

REFERENCES:

【1】Dranoff, G. Experimental mouse tumour models: what can be learnt about human cancer immunology? Nat Rev Immunol 12, 61-66 (2011).

【2】Kohnken, R., Porcu, P. & Mishra, A. Overview of the Use of Murine Models in Leukemia and Lymphoma Research. Front Oncol 7, 22 (2017).