top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hCRP mice
Strain Name C57BL/6-Crptm1(CRP)Bcgen/Bcgen Common Name  B-hCRP mice
Background C57BL/6 Catalog number 112500
Aliases 
PTX1

Model validation

from clipboard

Protein expression analysis in serum

from clipboard


Strain specific CRP expression analysis in wild-type C57BL/6 mice and homozygous B-hCRP mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCRP mice (H/H) (n=3, 6-week-old). Protein expression level of CRP was analyzed by ELISA. Mouse CRP was exclusively detectable in wild-type C57BL/6 mice. Human CRP was exclusively detectable in homozygous B-hCRP mice.

mRNA expression analysis

from clipboard


Strain specific analysis of CRP mRNA expression in wild-type C57BL/6 mice and B-hCRP mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCRP mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human Crp primers. Mouse Crp mRNA was detectable only in wild-type C57BL/6 mice. Human CRP mRNA was detectable only in homozygous B-hCRP mice but not in wild-type mice.