C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tfrctm1(TFRC)Bcgen/Bcgen • 113616
Product name | B-hCD3EDG/hTFR1 mice |
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Catalog number | 113616 |
Strain name | C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)BcgenCd3gtm1(CD3G)Bcgen Tfrctm1(TFRC)Bcgen/Bcgen |
Strain background | C57BL/6 |
NCBI gene ID | 916,915,917,7037 (Human) |
Aliases | IMD18, T3E, TCRE; CD3-DELTA, IMD19, T3D; CD3-GAMMA, IMD17, T3G; CD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90 |
Background:
Targeting strategy:
Verification:
Application:
This product is used for the pharmacological and safety evaluation of therapeutic drugs, as well as for the evaluation of pharmacodynamics and safety of treatments for tumors or neurodegenerative disease and the potential of drugs to penetrate the blood-brain barrier.
Strain specific TFR1 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3EDG/hTFR1 mice by flow cytometry. Bone marrow erythrocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD3EDG/hTFR1 mice (H/H, H/H). Protein expression was analyzed with anti-mouse TFR1 antibody (Biolegend, 113808) and anti-human TFR1 antibody (Biolegend, 334107) by flow cytometry. Mouse TFR1 was only detectable in wild-type C57BL/6JNifdc mice. Human TFR1 was exclusively detectable in homozygous B-hCD3EDG/hTFR1 mice, but not in wild-type C57BL/6JNifdc mice.
Strain specific CD3E expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3EDG/hTFR1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD3EDG/hTFR1 mice (H/H, H/H). Protein expression was analyzed with anti-mouse CD3E antibody (Biolegend, 100341) and anti-human CD3E antibody (BD Horizon™, 562426) by flow cytometry. Mouse CD3E was only detectable in wild-type C57BL/6JNifdc mice. Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hTFR1 mice.
Strain specific CD3E expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCD3EDG/hTFR1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD3EDG/hTFR1 mice (H/H, H/H). Protein expression was analyzed with anti-mouse CD3E antibody (Biolegend, 100341) and anti-human CD3E antibody (BD Horizon™, 562426) by flow cytometry. CD3E cannot be detected in B cells of wild-type C57BL/6JNifdc mice and homozygous B-hCD3EDG/hTFR1 mice.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6JNifdc mice (male, n=3, 9-week-old) and homozygous B-hCD3EDG/hTFR1 mice (male, n=3, 9-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, Neutrophils, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hTFR1 mice were similar to those in C57BL/6JNifdc mice. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from wild-type C57BL/6JNifdc mice (male, n=3, 9-week-old) and homozygous B-hCD3EDG/hTFR1 mice (male, n=3, 9-week-old). A. Flow cytometry analysis of the blood cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, Neutrophils, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hTFR1 mice were similar to those in C57BL/6JNifdc mice. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.
Frequency of leukocyte subpopulations in Lymph nodes by flow cytometry. Lymph nodes cells were isolated from wild-type C57BL/6JNifdc mice (male, n=3, 9-week-old) and homozygous B-hCD3EDG/hTFR1 mice (male, n=3, 9-week-old). A. Flow cytometry analysis of the lymph nodes cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, CD4+T cells, CD8+T cells and Tregs in B-hCD3EDG/hTFR1 mice were similar to those in C57BL/6JNifdc mice. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.001.