C57BL/6-Cfitm1(CFI)Bcgen/Bcgen • 112798
Gene targeting strategy for B-hCFI mice. The exons 1-14 of mouse Cfi gene that encode the whole molecule (ATG to STOP codon), including 3’UTR were replaced by human counterparts in B-hCFI mice. The promoter and 5’UTR region of the mouse gene are retained. The CFI expression is driven by the endogenous mouse Cfi promoter, while mouse Cfi gene transcription and translation will be disrupted.
Strain specific analysis of CFI mRNA expression in wild-type C57BL/6JNifdc mice and B-hCFI mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCFI mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CFI primers. Mouse Cfi mRNA was only detectable in wild-type mice. Human CFI mRNA was exclusively detectable in homozygous B-hCFI mice but not in wild-type mice.