Strain Name
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C57BL/6N-Gpr35tm1(GPR35)Bcgen/Bcgen
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Common Name
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B-hCCR3 mice
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Background
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C57BL/6N
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Catalog number
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111171
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Aliases
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NA
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mRNA and Protein expression analysis
Strain specific analysis of GPR35 gene expression in wild-type mice and B-hGPR35 mice by RT-PCR. Mouse Gpr35 mRNA was detectable in splenocytes of wild-type mice (+/+). Human GPR35 mRNA was detectable only in homozygous B-hGPR35 but not in wild-type mice.
Strain specific GPR35 expression analysis in homozygous B-hGPR35 mice by western blot. Stomach, liver and spleen were collected from wild-type mice (+/+) and homozygous B-hGPR35 mice (H/H), and analyzed by western blot with anti-GPR35 antibody. GPR35 was detectable in wild type mice and homozygous B-hGPR35 mice and MCF-7/RAW264.7 cell line, as the antibody is crossly reactive with GPR35 in human and mice.