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B-hAFP mice
Strain Name C57BL/6N-Afptm1(AFP)Bcgen/Bcgen  Common Name  B-hAFP mice
Background C57BL/6N Catalog number 111622
Aliases  AFP (AFPD, FETA, HPAFP)
mRNA and protein expression analysis

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Strain specific analysis of AFP gene expression in wild-type mice and B-hAFP mice by RT-PCR. Mouse Afp mRNA was detectable in fetal liver of wild-type mice (+/+). Human AFP mRNA was detectable only in homozygous B-hAFP mice (H/H) but not in wild-type mice.

Strain specific AFP expression analysis in homozygous B-hAFP mice by western blot. Fetal liver tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAFP mice (H/H), and analyzed by western blot with anti-hAFP antibody. Mouse AFP was detectable in wild-type mice. Human AFP was only detectable in homozygous B-hAFP mice but not in wild-type mice.