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B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice
Strain Name
C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen ,Tg(RP11-634C1)1Bcgen/Bcgen 
Common Name  B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice
Background C57BL/6 Catalog number 111954
Related Genes 

PD-1 (Programmed death-1) ;
CD274 (CD274 antigen);
LILRB2(ILT4, LIR2, CD85D, ILT-4, LIR-2, MIR10, MIR-10);
LILRB3(HL9, ILT5, LIR3, PIRB, CD85A, ILT-5, LIR-3, PIR-B, LILRA6);

Analysis of leukocytes cell subpopulation in spleen

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Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in spleen

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Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in lymph node 


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Analysis of lymph node leukocyte subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of T cells, B cells and NK cells in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in lymph node 

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Analysis of lymph node T cell subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in blood

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Analysis of blood leukocyte subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes and macrophages in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in blood

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Analysis of blood T cell subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.