top
Please input keywords
Order
*Country
한국
미국
일본
중국
영국
호주
프랑스
독일
이탈리아
싱가포르
인도
러시아
캐나다
스위스
기타 국가
*Province
*City
*Name
*Telephone
*Company
*Position
*Email
*Verification code
*Verification Code
B-hAPOE2 mice
Strain Name
C57BL/6JNifdc-Apoetm1(APOE*2)Bcgen/Bcgen
Common Name  B-hAPOE2 mice
Background C57BL/6JNifdc Catalog number 112562
Aliases 
APOE: AD2, LPG, APO-E, LDLCQ5
mRNA expression analysis

from clipboard


Strain specific analysis of APOE2 mRNA expression in wild-type C57BL/6J mice and B-hAPOE2 mice by RT-PCR. Brain RNA was isolated from wild-type C57BL/6J mice (+/+) and homozygous B-hAPOE2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human APOE primers. Mouse Apoe mRNA was detectable only in wild-type C57BL/6J mice. Human APOE2 mRNA was detectable only in homozygous B-hAPOE2 mice but not in wild-type mice. 

Protein expression analysis

from clipboard


Strain specific APOE2 expression analysis in homozygous B-hAPOE2 mice by ELISA. Serum was collected from wild-type mice C57BL/6 mice (+/+) and homozygous B-hAPOE2 mice (H/H), and analyzed by ELISA with species-specific APOE ELISA kit. Mouse APOE was detectable in wild-type mice (A). Human APOE2 was exclusively detectable in homozygous B-hAPOE2 mice but not in wild-type mice (B).