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B-hDLK1 mice
Strain Name C57BL/6-Dlk1tm1(DLK1)Bcgen/Bcgen Common Name  B-hDLK1 mice
Background C57BL/6N Catalog number 111435
Aliases 

DLK1: DLK, DLK-1, Delta1, FA1, PREF1, Pref-1, ZOG, pG2

Protein expression analysis  

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Western blot analysis of DLK1 protein expression in homozygous B-hDLK1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hDLK1 mice (H/H), and then analyzed by western blot with anti-DLK1 antibody. 1 μg/μL total proteins were loaded for western blotting analysis by JESS. Human DLK1 was detected in embryo, placenta, fetal liver and adrenal gland.

mRNA expression analysis

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Strain specific analysis of DLK1 mRNA expression in wild-type C57BL/6 mice and B-hDLK1 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hDLK1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human DLK1 primers. Mouse DLK1 mRNA were detectable in wild-type C57BL/6. Human DLK1 mRNA was detectable in homozygous B-hDLK1 mice.