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B-hITGA2 mice
Strain Name
C57BL/6-Itga2tm1(ITGA2)Bcgen/Bcgen
Common Name  B-hITGA2 mice
Background C57BL/6 Catalog number 111387
Aliases 
ITGA2: BR; GPIa; CD49B; HPA-5; VLA-2; VLAA2

Experimental results

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Experimental results Splenocytes-T cells


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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in T cells of wild-type mice, while hITGA2 was not detectable in T cells of homozygous mice. 

Experimental results Splenocytes-B cells

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in B cells of wild-type mice, while hITGA2 was not detectable in B cells of homozygous mice. 

Experimental results Splenocytes-NK cells

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in NK cells of wild-type mice, while hITGA2 was detectable in NK cells of homozygous mice. 

Experimental results Splenocytes-monocytes

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes of wild-type mice, while hITGA2 was detectable in monocytes of homozygous mice. 

Experimental results Splenocytes-macrophages

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in macrophages of wild-type mice, while hITGA2 was not detectable in macrophages of homozygous mice. 

Protein expression profile in splenocytes

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes and NK cells of wild-type mice, while hITGA2 was detectable in monocytes and NK cells of homozygous mice. 

Experimental results Bone marrow cells-B cells 

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in B cells of wild-type mice, while hITGA2 was not detectable in B cells of homozygous mice. 

Experimental results Bone marrow cells-NK cells 

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in NK cells of wild-type mice, while hITGA2 was detectable in NK cells of homozygous mice. 

Experimental results Bone marrow cells-monocytes 

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes of wild-type mice, while hITGA2 was detectable in monocytes of homozygous mice. 

Experimental results Bone marrow cells-macrophages 

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in macrophages of wild-type mice, while hITGA2 was not detectable in macrophages of homozygous mice. 

Protein expression profile in bone marrow cells

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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes and NK cells of wild-type mice, while hITGA2 was detectable in monocytes and NK cells of homozygous mice. 

Experimental results

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Experimental results Platelets


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Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Platelets were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in platelets of wild-type mice, while hITGA2 was detected in platelets of homozygous mice.