C57BL/6-Pdcd1tm1(PDCD1)BcgenCd274tm1(CD274)Bcgen,Tg(RP11-634C1)1Bcgen/Bcgen • 111954
| Product name | B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice |
|---|---|
| Catalog number | 111954 |
| Strain name | C57BL/6-Pdcd1tm1(PDCD1)BcgenCd274tm1(CD274)Bcgen,Tg(RP11-634C1)1Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 10288,11025,5133,29126 (Human) |
| Aliases | CD85D, ILT-4, ILT4, LIR-2, LIR2, MIR-10, MIR10; CD85A, HL9, ILT-5, ILT5, LILRA6, LIR-3, LIR3, PIR-B, PIRB; CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1; B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 |
Analysis of leukocytes cell subpopulation in spleen
Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated. B. Results of FACS analysis. The percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, and macrophages in B-hPD-1/hPD-L1,Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
Analysis of T cell subpopulation in spleen
Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated. B. Results of FACS analysis. The percentages of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1,Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
Analysis of leukocytes cell subpopulation in lymph node
Analysis of lymph node leukocyte subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated. B. Results of FACS analysis. The percentages of T cells, B cells, and NK cells in B-hPD-1/hPD-L1,Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
Analysis of T cell subpopulation in lymph node
Analysis of lymph node T cell subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated. B. Results of FACS analysis. The percentages of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1,Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
Analysis of leukocytes cell subpopulation in blood
Analysis of blood leukocyte subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1,Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. The percentages of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, and macrophages in B-hPD-1/hPD-L1,,Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
Analysis of T cell subpopulation in blood
Analysis of blood T cell subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and B-hPD-1/hPD-L1, Tg(hLILRB2/hLILRB3) mice(n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated. B. Results of FACS analysis. The percentages of CD8+ T cells, CD4+ T cells, and Tregs in B-hPD-1/hPD-L1,Tg(hLILRB2/hLILRB3) mice were similar to those in the C57BL/6 mice. Values are expressed as mean ± SEM.
