• The Cacng1 gene encodes a regulatory γ subunit of voltage-dependent Ca2+ channels.
• The genome sequences between exon 1 and exon 4 were depleted in B-Cacng1 KO mice, leading to the disruption of mouse Cacng1 gene.
• Mouse Cacng1 mRNA was only detectable in wild-type mice, but not in B-Cacng1 KO mice.
• This mouse model was a knock-out model, which can be used for the study of the function of CACNG1 in calcium ion channels as well as its functional diversity in different tissues.

Gene targeting strategy for B-Cacng1 KO mice. The genome sequences between exon 1 and exon 4 were depleted in B-Cacng1 KO mice, leading to the disruption of mouse Cacng1 gene.

Strain specific analysis of Cacng1 mRNA expression in wild-type C57BL/6JNifdc mice and B-CACNG1 KO mice by RT-PCR. Skeletal muscle and gastrocnemius RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-CACNG1 KO mice (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with two pairs of mouse Cacng1 primers. Mouse Cacng1 mRNA was only detectable in wild-type mice, but not in B-Cacng1 KO mice.