Description
- c-Rel is a member of the nuclear factor κB (NF-κB) transcription factor family. High levels of c-Rel expression are found primarily in B and T cells, with many c-Rel target genes involved in lymphoid cell growth and survival.
- In addition to c-Rel playing a major role in mammalian B and T cell function, the human c-Rel gene is a susceptibility locus for certain autoimmune diseases such as arthritis, psoriasis, and celiac disease.
- Gene editing strategy: The exons 1-10 of mouse Rel gene were knocked in B-Rel KO mice, resulting in a disruption of the Rel gene.
- Protein expression analysis: REL protein was detected in the spleen, thymus and heart of wild-type mice, but not in homozygous B-Rel KO mice.
- Application: This product is used for the development of drugs for treating tumors and basic research.
Targeting strategy
Gene targeting strategy for B-Rel KO mice. The exons 1-10 of mouse Rel gene were knocked out in B-Rel KO mice, resulting in a disruption of the Rel gene.
Protein expression analysis
Western blot analysis of REL protein expression in homozygous B-Rel KO mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-Rel KO mice (-/-), and then analyzed by western blot with Anti-c-Rel antibody (Abcam, ab309131) . 50 μg total proteins were loaded for western blotting analysis. REL protein was detected in the spleen, thymus and heart of wild-type mice, but not in homozygous B-Rel KO mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Rel KO mice] (Cat# 114010) was purchased from Biocytogen.
