Strain specific GDF8 expression analysis in homozygous B-hGDF8 mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), and analyzed by ELISA. Human and mouse GDF8 proteins were both detectable in wild-type C57BL/6 and B-hGDF8 mice. The ELISA kit cross recognizes human and mouse GDF8.

Strain specific analysis of GDF8 mRNA expression in wild-type C57BL/6 mice and B-hGDF8 mice by RT-PCR. Skeletal muscle RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GDF8 primers. Mouse Gdf8 mRNA was detectable only in wild-type C57BL/6 mice. Human GDF8 mRNA was detectable only in homozygous B-hGDF8 mice but not in wild-type mice.