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B-hGDF8 mice
Strain Name
 C57BL/6N-Gdf8tm1(GDF8)Bcgen/Bcgen 
Common Name  B-hGDF8 mice
Background C57BL/6N Catalog number 112413
Aliases 

GDF8: MSLHP, MSTN

Protein expression analysis

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Strain specific GDF8 expression analysis in homozygous B-hGDF8 mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), and analyzed by ELISA. Human and mouse GDF8 proteins were both detectable in wild-type C57BL/6 and B-hGDF8 mice. The ELISA kit cross recognizes human and mouse GDF8.

mRNA expression analysis

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Strain specific analysis of GDF8 mRNA expression in wild-type C57BL/6 mice and B-hGDF8 mice by RT-PCR. Skeletal muscle RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GDF8 primers. Mouse Gdf8 mRNA was detectable only in wild-type C57BL/6 mice. Human GDF8 mRNA was detectable only in homozygous B-hGDF8 mice but not in wild-type mice.