B-hGDF8 mice

C57BL/6N-Gdf8tm1(GDF8)Bcgen/Bcgen • 112413

제품 주문
B-hGDF15 mice
B-hGEP mice

B-hGDF8 mice

Product nameB-hGDF8 mice
Catalog number112413
Strain nameC57BL/6N-Gdf8tm1(GDF8)Bcgen/Bcgen
Strain backgroundC57BL/6N
NCBI gene ID2660 (Human)
AliasesGDF8, MSLHP
제품 주문

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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hGDF8 mice. The exons 1-3 of mouse Gdf8 gene that encodes the full-length protein were replaced by human GDF8 exons 1-3 in B-hGDF8 mice.

      Protein expression analysis

      Strain specific GDF8 expression analysis in homozygous B-hGDF8 mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), and analyzed by ELISA. GDF8 proteins were both detectable in wild-type C57BL/6 and B-hGDF8 mice, as the ELISA kit cross recognized human and mouse GDF8.

      mRNA expression analysis
      Strain specific analysis of GDF8 mRNA expression in wild-type C57BL/6 mice and B-hGDF8 mice by RT-PCR. Skeletal muscle RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GDF8 primers. Mouse Gdf8 mRNA was detectable only in wild-type C57BL/6 mice. Human GDF8 mRNA was detectable only in homozygous B-hGDF8 mice but not in wild-type mice.