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B-hPD-1/hPD-L1/hOX40 mice
Strain Name
C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Tnfrsf4tm1(TNFRSF4)Bcgen/Bcgen
Common Name  B-hPD-1/hPD-L1/hOX40 mice
Background C57BL/6 Catalog number  311112
Related Genes 

PD-1(Programmed death-1) 

CD274 (CD274 antigen)

TNFRSF4(Tumor necrosis factor receptor superfamily, member 4, also known as OX40

NCBI Gene ID
18566,60533,22163

Targeting strategy


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Gene targeting strategy for B-hPD-1/hPD-L1/hOX40 mice. 

The exon 2 of mouse PD-1 gene that encodes the extracellular domain was replaced by human PD-1 exon 2 in B-hPD-1/hPD-L1/hOX40 mice. The exon 3 of mouse pdl1 gene that encode the extracellular domain was replaced by human PD-L1 exon 3 in B-hPD-1/hPD-L1/hOX40 mice. The exons 1-5 of mouse Tnfrsf4 gene that  encode the extracellular domain were replaced by human TNFRSF4  exons 1-5 in B-hPD-1/hPD-L1/hOX40 mice . 


Protein expression analysis


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Strain specific PD-1, PD-L1 and OX40 expression analysis in homozygous B-hPD-1/hPD-L1/hOX40 mice by flow cytometry. 

Splenocytes were collected from WT and homozygous B-hPD-1/hPD-L1/hOX40 (H/H) mice analyzed by flow cytometry with species-specific anti-PD-1 antibody, anti-PD-L1 antibody and anti-OX40 antibody. Mouse PD-1, PD-L1 and OX40 were detectable in WT mice. Human PD-1, PD-L1 and OX40 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hOX40 but not WT mice. 


from clipboard


Strain specific PD-1, PD-L1 and OX40 expression analysis in homozygous B-hPD-1/hPD-L1/hOX40 mice by flow cytometry.

Splenocytes were collected from WT and homozygous B-hPD-1/hPD-L1/hOX40 (H/H) mice mice after stimulation with anti-CD3ε in vivo (7.5 μg/mice) and analyzed by flow cytometry with species-specific anti-PD-1 antibody, anti-PD-L1 antibody and anti-OX40 antibody. Mouse PD-1, PD-L1 and OX40 were detectable in WT mice. Human PD-1, PD-L1 and OX40 were exclusively detectable in homozygous B-hPD-1/hPD-L1/hOX40 but not WT mice.