| Strain Name |
C57BL/6-Cd33tm1(CD33)Bcgen/Bcgen
|
Common Name | B-hCD33 mice |
| Background | C57BL/6 | Catalog number |
110823 |
|
Related Genes |
SIGLEC-3, SIGLEC3, p67 | ||
|
NCBI Gene ID |
12489 | ||
mRNA expression analysis
Strain specific analysis of CD33 gene expression in wild-type mice and B-hCD33 mice by RT-PCR. Mouse CD33 mRNA was detectable in splenocytes of wild-type mice (+/+). Human CD33 mRNA was detectable only in homozygous B-hCD33 but not in wild-type mice.
Protein expression analysis in macrophages in bone marrow
Strain specific CD33 expression analysis in homozygous B-hCD33 mice by flow cytometry. Bone marrow cells were collected from wild-type mice (+/+) and homozygous B-hCD33 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in wild-type mice. Human CD33 was exclusively detectable in homozygous B-hCD33 but not in wild-type mice.
Strain specific CD33 expression analysis in homozygous B-hCD33 mice by flow cytometry. Bone marrow cells were collected from wild-type mice (+/+) and homozygous B-hCD33 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD33 antibody. Mouse CD33 was detectable in wild-type mice. Human CD33 was exclusively detectable in homozygous B-hCD33 but not in wild-type mice.
