Description 

The mouse B7-h3 gene was replaced by human B7-H3 coding sequence in B-hB7-H3 CT26.WT cells. Human B7-H3 is highly expressed on the surface of B-hB7-H3 CT26.WT cells.

Application
B-hB7-H3 CT26.WT cells have the capability to establish tumors in vivo and can be used for efficacy studies.
Targeting strategy
Gene targeting strategy for B-hB7-H3 CT26.WT cells. The chemic human and mouse B7-H3 coding sequence was inserted to replace part of murine exon 3 and exon 4. The insertion disrupts the endogenous murine B7-h3 gene, resulting in a non-functional transcript.
Protein expression analysis 

B7-H3 expression analysis in B-hB7-H3 CT26.WT cells by flow cytometry. Single cell suspensions from B-hB7-H3 CT26.WT cultures were stained with species-specific anti-B7-H3 antibody. Human B7-H3 was detected on the surface of B-hB7-H3 CT26.WT cells but not wild-type CT26.WT cells. The 3-H02 clone of B-hB7-H3 CT26.WT cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

Subcutaneous homograft tumor growth of B-hB7-H3 CT26.WT cells. B-hB7-H3 CT26.WT cells and wild-type CT26.WT cells were subcutaneously implanted into BALB/c mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-hB7-H3 CT26.WT cells were able to establish tumors in vivo and can be used for efficacy studies.

Protein expression analysis of tumor cells