Gene editing strategy
An F2A-iCre sequence cassette was placed between the coding sequence of exon 3 and 3’UTR of the Grpr gene in C57BL/6 ES cells. This strain was maintained on a C57BL/6 genetic background.
Phenotype Analysis
(I) Schematic depicting virus and retrobeads injection, as well as recording configuration in spinal slices. (J) All recorded cells were filled with biocytin (blue) and were beads-positive (red). GRPR+ fibers were labeled with EYFP (green). Scale bar, 10 mm.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Grpr-iCre mice] (Cat# 110125) was purchased from Biocytogen.
