• The activation of CD30 is mediated by the CD30 ligand (CD30-L), resulting in the formation of a homo-trimer, which recruits TNF receptor-associated factors (TRAF) and subsequently activates the downstream signaling pathways of NF-κB and MAPK/ERK.
• The genome of the mouse Cd30 gene encoding the extracellular domain was replaced with human CD30 counterpart in B-hCD30/hCD30L mice. The genome of the mouse Cd30l gene encoding the extracellular domain was replaced with human CD30L counterpart in B-hCD30/hCD30L mice.
• Mouse Cd30 and Cd30l  mRNA were detectable in wild-type mice but not in homozygous B-hCD30/hCD30L mice. Human CD30 and CD30L mRNA were detectable in homozygous B-hCD30/hCD30L mice but not in wild-type mice.
• Human CD30 and CD30L protein were only detectable in homozygous B-hCD30/hCD30L mice but not in wild-type mice.
• Application: This product is used for pharmacodynamics and safety evaluation of  drugs.

Gene targeting strategy for B-hCD30/hCD30L mice.

The exons 1-9 of mouse Cd30 gene that encode the extracellular domain were replaced by human CD30 exons 1-11 in B-hCD30/hCD30L mice. The genomic region of mouse Cd30 gene that encodes transmembrane domain and cytoplasmic portion was retained. The promoter and 5’UTR region of the mouse gene were also retained. The 3’UTR region of the mouse gene are replaced by human counterparts. The CD30 expression was driven by endogenous mouse Cd30 promoter, while mouse Cd30 gene transcription and translation will be disrupted.

The exons 1-4 of mouse Cd30l gene that encode extracellular domain were replaced by human counterparts in B-hCD30/hCD30L mice. The genomic region of mouse Cd30l gene that encodes transmembrane domain and cytoplasmic portion was retained. The promoter and 5’UTR region of the mouse gene were also retained. The 3’UTR region of the mouse gene are replaced by human counterparts. The CD30L expression was driven by endogenous mouse Cd30l promoter, while mouse Cd30l gene transcription and translation will be disrupted.

Species specific analysis of CD30 and CD30L gene expression in wild-type C57BL/6JNifdc mice and homozygous B-hCD30/hCD30L mice by RT-PCR. Spleen was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD30/hCD30L mice (H/H;H/H). Mouse Cd30 and Cd30l mRNA were detectable in wild-type C57BL/6JNifdc mice. Human CD30 and CD30L mRNA were detectable only in homozygous B-hCD30/hCD30L mice, but not in wild-type C57BL/6JNifdc mice.

Strain specific CD30 expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hCD30/hCD30L mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD30/hCD30L mice (H/H;H/H). Splenocytes were incubated in a medium containing Cell Activation Cocktail (without Brefeldin A) in vitro for 20 h, protein expression was analyzed with anti-mouse CD30 antibody (Biolegend, 102306) and anti-human CD30 antibody (Biolegend, 333909) by flow cytometry. Mouse/human CD30 was detectable in wild-type C57BL/6JNifdc mice and homozygous B-hCD30/hCD30L mice, as the anti-mouse CD30 antibody was cross-reactive between mouse and human. Human CD30 was detectable in homozygous B-hCD30/hCD30L mice.

Strain specific CD30L expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hCD30/hCD30L mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCD30/hCD30L mice (H/H;H/H). Splenocytes were incubated in a medium containing Cell Activation Cocktail (without Brefeldin A) in vitro for 20 h, protein expression was analyzed with anti-mouse CD30L antibody (Biolegend, 106405) and anti-human CD30L antibody (RD, FAB1028A) by flow cytometry. Mouse CD30L was detectable in wild-type C57BL/6JNifdc mice. Human CD30L was detectable in homozygous B-hCD30/hCD30L mice.