C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)Bcgen Cd19tm4(CD19)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen • 113343
Product name | B-hCD3EDG/hCD19/hBCMA mice |
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Catalog number | 113343 |
Strain name | C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)Bcgen Cd19tm4(CD19)Bcgen Tnfrsf17tm1(TNFRSF17)Bcgen/Bcgen |
Strain background | C57BL/6 |
NCBI gene ID | 916,915,917,930,608 (Human) |
Aliases | IMD18, T3E, TCRE; CD3-DELTA, IMD19, T3D; CD3-GAMMA, IMD17, T3G; B4, CVID3; BCM, BCMA, CD269, TNFRSF13A |
Strain specific analysis of BCMA mRNA expression in wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19/hBCMA mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCD3EDG/hCD19/hBCMA mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse and human primers. Mouse Bcma mRNA were detectable only in wild-type mice. Human BCMA mRNA were detectable only in B-hCD3EDG/hCD19/hBCMA mice but not in wild-type mice.
Strain specific CD3E expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hCD19/hBCMA mice by flow cytometry. Splenocytes (A) and blood cells (B) were collected from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19/hBCMA mice (female, 8-week-old, n=1). Protein expression was analyzed with anti-mouse CD3E antibody (Biolegend, 100312) and anti-human CD3E antibody (BD Horizon™, 562426) by flow cytometry. Mouse CD3E was only detectable in wild-type C57BL/6 mice. Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hCD19/hBCMA mice, but not in wild-type C57BL/6 mice.
Strain specific CD19 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hCD19/hBCMA mice by flow cytometry. Splenocytes (A) and blood cells (B) were collected from wild-type C57BL/6 mice and homozygous B-hCD3EDG/hCD19/hBCMA mice (female, 8-week-old, n=1). Protein expression was analyzed with anti-mouse CD19 antibody (Biolegend, 115507) and anti-human CD19 antibody (Biolegend, 392503) by flow cytometry. Mouse CD19 was only detectable in wild-type C57BL/6 mice. Human CD19 was exclusively detectable in homozygous B-hCD3EDG/hCD19/hBCMA mice, but not in wild-type C57BL/6 mice.
Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild-type C57BL/6 mice (female, n=3, 8-week-old) and homozygous B-hCD3EDG/hCD19/hBCMA mice (female, n=3, 8-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hCD19/hBCMA mice were similar to those in wild-type C57BL/6 mice. Values are expressed as mean ± SEM.
Frequency of leukocyte subpopulations in blood by flow cytometry. Blood cells were isolated from wild-type C57BL/6 mice (female, n=3, 8-week-old) and homozygous B-hCD3EDG/hCD19/hBCMA mice (female, n=3, 8-week-old). A. Flow cytometry analysis of the blood was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hCD19/hBCMA mice were similar to those in wild-type C57BL/6 mice. Values are expressed as mean ± SEM.
Frequency of leukocyte subpopulations in lymph nodes by flow cytometry. Lymph nodes cells were isolated from wild-type C57BL/6 mice (female, n=3, 8-week-old) and homozygous B-hCD3EDG/hCD19/hBCMA mice (female, n=3, 8-week-old). A. Flow cytometry analysis of the Lymph nodes cells was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, CD4+ T cells, CD8+ T cells and Tregs in B-hCD3EDG/hCD19/hBCMA mice were similar to those in wild-type C57BL/6 mice.