Description
- Background: MMP7 is mostly expressed in epithelial cells, which is secreted as a zymogen. In activated alveolar epithelial cells, MMP-7 cuts osteopontin (OPN) and enhances its function, thereby upregating and activating MMP-7. In this pro-fibrotic effect, osteopontin upregates the expression of ECM (extracellular matrix) proteins. Various MMPs are involved in the process of pulmonary fibrosis.
- Application: This product is used for pharmacodynamics and safety evaluation of IPF (idiopathic pulmonary fibrosis) and other diseases.
- Targeting strategy: The exons 1~6 of mouse Mmp7 gene that encode the full-length protein and 3’UTR were replaced by human MMP7 in B-hMMP7 mice.
- Validation: Mouse MMP7 was detectable in wild-type C57BL/6. Human MMP7 was exclusively detectable in homozygous B-hMMP7 mice
Targeting strategy
Gene targeting strategy for B-hMMP7 mice. The exons 1~6 of mouse Mmp7 gene that encode the full-length protein and 3’UTR were replaced by human MMP7 exons 1~6 in B-hMMP7 mice.
Protein expression analysis in serum
Strain specific MMP7 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hMMP7 mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) (Female, 8-weeks-old, n=3) and homozygous B-hMMP7 mice (H/H) (Female, 8-weeks-old, n=3). Protein expression level of MMP7 was analyzed by ELISA (Mouse MMP-7 ELISA Kit (Colorimetric), Novus Biologicals, NBP3-06895; Human Total MMP-7 Quantikine ELISA Kit, R&D, DMP700). Mouse MMP7 was detectable in wild-type C57BL/6 mice. Human MMP7 was exclusively detectable in homozygous B-hMMP7 mice. Values are expressed as mean ± SEM.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hMMP7 mice] (Cat# 112823) was purchased from Biocytogen.
