Targeting strategy
Gene targeting strategy for B-hK5-hARx2 mice. The full coding sequences of human AR gene, driven by the human Keratin 5 promoter, was inserted into mouse Hipp11 (H11) locus in B-hK5-hARx2 mice.
mRNA Expression Analysis
Strain specific analysis of AR mRNA expression homozygous B-hK5-hARx2 mice by RT-PCR. Skin RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous female B-hK5-hARx2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human AR primers. Human AR mRNA was exclusively detectable in homozygous B-hK5-hARx2 mice but not in wild-type mice.
Protein Expression Analysis
Western blot analysis of Androgen Receptor protein expression in homozygous B-hK5-hARx2 mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hK5-hARx2 mice (H/H), and then analyzed by western blot with human-specific anti-androgen receptor antibody (CST, #5153). 40 μg total proteins were loaded for western blot analysis. Androgen receptor was only detected in dorsal skin and abdominal skin from homozygous B-hK5-hARx2 mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hK5-hAR×2 mice] (Cat# 113202) was purchased from Biocytogen.
