• Integrin α4β1 is composed of ITGA4 and ITGB1 heterodimer. α4β1 can bind to VCAM-1 and is expressed in most leukocytes. The α4β1 integrin plays a role in cell adhesion, migration and movement. Additionally, α4β1 is involved in the homing of memory and effector T lymphocytes to inflammatory tissues, such as intestinal tissues.
• The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hα4β1 mice. The exons 2-15 of mouse Itgb1 gene that encode the extracellular domain were replaced by human ITGB1 exons 2-15 in B-hα4β1 mice.
• Mouse ITGA4 and ITGB1 were only detectable in wild-type C57BL/6JNifdc mice. Human ITGA4 and ITGB1 were exclusively detectable in homozygous B-hα4β1 mice.
• Application: This product is used for pharmacological and safety evaluation of autoimmune diseases such as Inflammatory bowel disease, psoriasis and arthritis.

Gene targeting strategy for B-hα4β1 mice.

The exons 2-27 of mouse Itga4 gene that encode the extracellular domain were replaced by human ITGA4 exons 2-27 in B-hα4β1 mice.

The exons 2-15 of mouse Itgb1 gene that encode the extracellular domain were replaced by human ITGB1 exons 2-15 in B-hα4β1 mice.

Strain specific ITGA4 and ITGB1 expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice. Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse ITGB1 antibody (Biolegend, 102215), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB1 antibody (Biolegend, 303003) by flow cytometry. Mouse ITGA4 and ITGB1 were only detectable in wild-type C57BL/6JNifdc mice. Human ITGA4 and ITGB1 were exclusively detectable in homozygous B-hα4β1 mice, but not in wild-type C57BL/6JNifdc mice.

Strain specific ITGA4 and ITGB1 expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice. Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse ITGB1 antibody (Biolegend, 102215), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB1 antibody (Biolegend, 303003) by flow cytometry. Mouse ITGA4 and ITGB1 were only detectable in wild-type C57BL/6JNifdc mice. Human ITGA4 and ITGB1 were exclusively detectable in homozygous B-hα4β1 mice, but not in wild-type C57BL/6JNifdc mice.

Strain specific ITGA4 and ITGB1 expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice. Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse ITGB1 antibody (Biolegend, 102215), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB1 antibody (Biolegend, 303003) by flow cytometry. Mouse ITGA4 and ITGB1 were only detectable in wild-type C57BL/6JNifdc mice. Human ITGA4 and ITGB1 were exclusively detectable in homozygous B-hα4β1 mice, but not in wild-type C57BL/6JNifdc mice.

Strain specific ITGA4 and ITGB1 expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice. Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse ITGB1 antibody (Biolegend, 102215), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB1 antibody (Biolegend, 303003) by flow cytometry. Mouse ITGA4 and ITGB1 were only detectable in wild-type C57BL/6JNifdc mice. Human ITGA4 and ITGB1 were exclusively detectable in homozygous B-hα4β1 mice, but not in wild-type C57BL/6JNifdc mice.

Strain specific ITGA4 and ITGB1 expression analysis in wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6JNifdc mice and homozygous B-hα4β1 mice. Protein expression was analyzed with anti-mouse ITGA4 antibody (Biolegend, 103705), anti-mouse ITGB1 antibody (Biolegend, 102215), anti-human ITGA4 antibody (Biolegend, 304307) anti-human ITGB1 antibody (Biolegend, 303003) by flow cytometry. Mouse ITGA4 and ITGB1 were only detectable in wild-type C57BL/6JNifdc mice. Human ITGA4 and ITGB1 were exclusively detectable in homozygous B-hα4β1 mice, but not in wild-type C57BL/6JNifdc mice.