C57BL/6-Cd3etm2(CD3E)Bcgen Ms4a1tm2(MS4A1)Bcgen/Bcgen • 121703
| Product name | B-hCD3EDG/hCD20 mice |
|---|---|
| Catalog number | 121703 |
| Strain name | C57BL/6-Cd3etm2(CD3E)Bcgen Ms4a1tm2(MS4A1)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 915,917,916,931 (Human) |
| Aliases | CD3-DELTA, IMD19, T3D; CD3-GAMMA, IMD17, T3G; IMD18, T3E, TCRE; B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7 |
Targeting strategyGene targeting strategy for B-hCD3EDG/hCD20 mice.
The exons 2-8 of mouse Cd3e gene were replaced by human CD3E exons 2-9 in B-hCD3EDG/hCD20 mice. The exons 1-5 of mouse Cd3d and the exons 1-6 of Cd3g gene were replaced by human CD3D exons 1-5 and CD3G exons 1-7 in B-hCD3EDG/hCD20 mice, respectively. The exons 2-7 of mouse Cd20 gene that encode the full-length protein were replaced by human CD20 exons 2-7 in B-hCD3EDG/hCD20 mice.
mRNA expression analysis
Strain specific analysis of CD3DG gene expression in wild type (WT) mice and B-hCD3EDG/hCD20 mice by RT-PCR. Mouse Cd3d and Cd3g mRNA were detectable only in thymocytes of WT mice (+/+). Human CD3D and CD3G mRNA were detectable only in homozygous B-hCD3EDG/hCD20 mice (H/H;H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hCD3EDG/hCD20 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hCD20 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hCD20 mice (H/H;H/H) but not in WT mice (+/+).
Strain specific CD20 expression analysis in homozygous B-hCD3EDG/hCD20 mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hCD20 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD20 antibody. Mouse CD20 was detectable in WT mice (+/+). Human CD20 was exclusively detectable in homozygous B-hCD3EDG/hCD20 mice (H/H;H/H) but not in WT mice (+/+).
