C57BL/6-Cd44tm1(CD44)Bcgen/Bcgen • 110961
Targeting strategyGene targeting strategy for B-hCD44 mice.
The exons 2-17 of mouse Cd44 gene that encode the extracellular domain were replaced by human CD44 exons 2-16 in B-hCD44 mice.
Protein expression analysis in T cells
Strain specific CD44 expression analysis in heterozygous B-hCD44 mice by flow cytometry.
Splenocytes were collected from WT and heterozygous B-hCD44 (H/+) mice, and analyzed by flow cytometry with species-specific anti-CD44 antibody. Mouse CD44 was detectable in WT and heterozygous B-hCD44 mice. Human CD44 was exclusively detectable in heterozygous B-hCD44 but not WT mice.
Protein expression analysis in B cells
Strain specific CD44 expression analysis in heterozygous B-hCD44 mice by flow cytometry.
Splenocytes were collected from WT and heterozygous B-hCD44 (H/+) mice, and analyzed by flow cytometry with species-specific anti-CD44 antibody. Mouse CD44 was detectable in WT and heterozygous B-hCD44 mice. Human CD44 was exclusively detectable in heterozygous B-hCD44 mice but not in WT mice.
