IL17A
The exons 1-3 of mouse Il17a gene that encode the extracellular domain were replaced by human IL17A exons 1-3 in B-hIL17A/hIL17F/hIL17RA/hIL17RC mice.
The endogenous mouse promoter, 5′ UTR, and 3′ UTR regions are retained, allowing human IL17A expression to be driven by the native mouse Il17a promoter, while endogenous mouse Il17a transcription and translation are abolished.
IL17F
The exons 2-3 of mouse Il17f gene that encode full-length protein were replaced by human IL17F exons 2-3 in B-hIL17A/hIL17F/hIL17RA/hIL17RC mice.
The endogenous mouse promoter, 5′ UTR, and 3′ UTR regions are retained, allowing human IL17F expression to be driven by the native mouse Il17f promoter, while endogenous mouse Il17f transcription and translation are abolished.
IL17RA
The exons 2-9 of mouse Il17ra gene that encode the extracellular domain were replaced by human IL17RA exons 2-9 in B-hIL17A/hIL17F/hIL17RA/hIL17RC mice.
The endogenous mouse promoter, 5′ UTR, and 3′ UTR regions are retained, allowing human IL17RA expression to be driven by the native mouse Il17ra promoter, while endogenous mouse Il17ra transcription and translation are abolished.
IL17RC
The Chimeric IL17RC CDS CDS that encodes human IL17RC signal peptide and extracellular domain, mouse Il17rc transmembrane and cytoplasmic domain was inserted after the 5’UTR of mouse IL17rc gene in B-hIL17A/hIL17F/hIL17RA/hIL17RC mice.
The chimeric IL17RC protein expression will be driven by endogenous mouse Il17rc promoter, while mouse Il17rc gene transcription and translation will be disrupted.
