B-hSIRPA/hCD47,Prkdc/Il2rg KO mice

C57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Prkdctm1Bcgen Il2rgtm1Bcgen/Bcgen • 140739

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B-hSIRPA/hCD47,Prkdc/Il2rg KO mice

Product nameB-hSIRPA/hCD47,Prkdc/Il2rg KO mice
Catalog number140739
Strain nameC57BL/6-Sirpatm1(SIRPA)Bcgen Cd47tm1(CD47)Bcgen Prkdctm1Bcgen Il2rgtm1Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID140885,23543,3561,5591,961,16186,19090,93686,19261,16423 (Mouse)
AliasesBIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP; CD132; CIDX; IL-2RG; IMD4; P64; SCIDX; SCIDX1; DNA-PKC; DNA-PKcs; DNAPK; DNAPKc; DNPK1; HYRC; HYRC1; IMD26; XRCC7; p350; FOX2; Fox-2; HNRBP2; HRNBP2; RBM9; RTA; dJ106I20.3; fxh; IAP; MER6; OA3; gc; p64; [g]c; gamm; CD132; gamma(; gamma(c); DNA; DOX; dxn; DNA-; XRCC; p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs;AI326420; AU019811; DNA-PKcs; Fbm2; Fxh; Hrnbp2; Rbm9; Bi; P8; Pt; Bit; P84; SHP; SIRP; CD172; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480; It; IAP; Itgp; AA407862; AI848868; AW108519; 9130415E20Rik; B430305P08Rik
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  • Phenotypic analysis

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      Protein expression analysis
      Species specific SIRPα and CD47 expression analysis in B-hSIRPA/hCD47,Prkdc/Il2rg KO mice by flow cytometry. Splenocytes isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (H/H) were analyzed by flow cytometry with anti-SIRPα and anti-CD47 antibodies. Human SIRPα and CD47 were exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.

      Species specific SIRPα and CD47 expression analysis in B-hSIRPA/hCD47,Prkdc/Il2rg KO mice by flow cytometry. Splenocytes were isolated from C57BL/6 (+/+) mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (H/H) stimulated with anti-mCD3ε in vivo and were analyzed by flow cytometry with anti-SIRPα and anti-CD47 antibodies. Human SIRPα and CD47 were exclusively detectable in homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice but not in C57BL/6 mice. Mouse SIRPα was detectable in C57BL/6 mice and homozygous B-hSIRPA/hCD47,Prkdc/Il2rg KO mice, indicating that this anti-mouse SIRPα antibody was cross-reacting with human SIRPα.

      Analysis of leukocyte subpopulations in spleen

      Analysis of leukocyte subpopulations in spleen by FACS. Splenocytes were isolated from C57BL/6, B-NDG mice and B-hSIRPA/hCD47,Prkdc/Il2rg KO mice (n=3, 11-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. Single live cells were gated for CD45 population and used for further analysis as indicated here. T, B and NK cells were undetectable in B-NDG mice and B-hSIRPA/hCD47,Prkdc/Il2rg KO mice.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hSIRPA/hCD47,Prkdc/Il2rg KO mice] (Cat# 140739) was purchased from Biocytogen.