Targeting strategy
Gene targeting strategy for B-Tg(hIL8) mice. The BAC containing the whole human IL8 genome sequence was randomly inserted into mouse genome in B-Tg(hIL8) mice.
Protein expression analysis (F1)
Strain specific IL8 expression analysis in B-Tg(hIL8) mice by ELISA. Serum were collected from wild-type C57BL/6 mice and B-Tg(hIL8) mice after stimulated with LPS in vivo, and analyzed by ELISA with species-specific IL8 ELISA kit. Human IL8 was exclusively detectable in B-Tg(hIL8) mice.
Protein expression analysis (F2)
Strain specific IL8 expression analysis in B-Tg(hIL8) mice by ELISA. Serum were collected from wild-type C57BL/6 mice and B-Tg(hIL8) mice after stimulated with LPS in vivo, and analyzed by ELISA with species-specific IL8 ELISA kit. Human IL8 was exclusively detectable in B-Tg(hIL8) mice.
Protein expression analysis (F3)
Strain specific IL8 expression analysis in B-Tg(hIL8) mice by ELISA. Serum were collected from wild-type C57BL/6 mice and B-Tg(hIL8) mice after stimulated with LPS in vivo, and analyzed by ELISA with species-specific IL8 ELISA kit. Human IL8 was exclusively detectable in B-Tg(hIL8) mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Tg(hIL8) mice] (Cat# 110832) was purchased from Biocytogen.
