B-NDG hCD98HC mice

NOD.CB17-Prkdcscid Il2rgtm1Bcgen Slc3a2tm1(SLC3A2)Bcgen/Bcgen • 113117

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B-NDG hCD98HC mice

Product nameB-NDG hCD98HC mice
Catalog number113117
Strain nameNOD.CB17-Prkdcscid Il2rgtm1Bcgen Slc3a2tm1(SLC3A2)Bcgen/Bcgen
Strain backgroundB-NDG
NCBI gene ID6520 (Human)
Aliases4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE
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이 페이지에서

  • Targeting strategy
  • Phenotypic analysis
  • Physiological data

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    출판물

      Targeting Strategy

      The extracellular domain of the mouse Cd98 gene (exons 2–10) is replaced with human CD98 exons 4–12.

      Human CD98HC mRNA Expression

      Species specific CD98HC gene expression in wild-type B-NDG mice and homozygous humanized B-NDG hCD98HC mice by RT-PCR. Kidney and brain were collected from wild-type B-NDG mice (+/+) and homozygous B-NDG hCD98HC mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CD98HC primers.

      Human CD98HC Protein Expression in spleen

      Strain specific CD98HC expression analysis in wild-type B-NDG mice and homozygous humanized B-NDG hCD98HC mice. Splenocytes were collected from wild-type B-NDG mice (+/+) and homozygous B-NDG hCD98HC mice (H/H). Protein expression was analyzed with anti-mouse CD98HC antibody (Biolegend, 128211) and anti-human CD98HC antibody (Biolegend, 315603) by flow cytometry. Mouse CD98HC was detectable in neutrophils, DCs, monocytes and macrophages of wild-type B-NDG mice, and human CD98HC was exclusively detectable in homozygous B-NDG hCD98HC mice.

      Human CD98HC Protein Expression in BM

      Strain specific CD98HC expression analysis in wild-type B-NDG mice and homozygous humanized B-NDG hCD98HC mice. Bone marrow cells were collected from wild-type B-NDG mice (+/+) and homozygous B-NDG hCD98HC mice (H/H). Protein expression was analyzed with anti-mouse CD98HC antibody (Biolegend, 128211) and anti-human CD98HC antibody (Biolegend, 315603) by flow cytometry. Mouse CD98HC was detectable in neutrophils, DCs, monocytes and macrophages of wild-type B-NDG mice, and human CD98HC was exclusively detectable in homozygous B-NDG hCD98HC mice.

      Human CD98HC Protein Expression Across Tissues

      Western blot analysis of hCD98HC protein expression in homozygous B-NDG hCD98HC mice. Various tissue lysates were collected from wild-type B-NDG and homozygous B-NDG hCD98HC mice (H/H), and then analyzed by western blot with species-specific anti-human CD98 antibody (Abcam, ab307587). 40 μg total proteins were loaded for western blotting analysis.

      Normal Hematological Profiles

      Complete blood count (CBC) of B-NDG hCD98HC mice. CD98HC humanization does not disrupt hematopoiesis or immune cell composition. Values are expressed as mean ± SD.

      Normal Liver and Kidney Function Profiles

      Biochemical test of B-NDG hCD98HC mice. CD98HC humanization does not adversely affect metabolic health or organ function. Values are expressed as mean ± SD.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-NDG hCD98HC mice] (Cat# 113117) was purchased from Biocytogen.