NOD.CB17-PrkdcscidIl2rgtm1BcgenIl3tm1(IL3)BcgenCsf2tm1(CSF2)BcgenCsf1tm1(CSF1)BcgenThpotm1(THPO)Bcgen/Bcgen • 112479
| Product name | huHSC-B-NDG MGMT3 mice |
|---|---|
| Catalog number | 112479 |
| Strain name | NOD.CB17-PrkdcscidIl2rgtm1BcgenIl3tm1(IL3)BcgenCsf2tm1(CSF2)BcgenCsf1tm1(CSF1)BcgenThpotm1(THPO)Bcgen/Bcgen |
| Strain background | B-NDG |
| NCBI gene ID | 12981,12977,16187,21832,16186,19090 (Human) |
| Aliases | CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM; op; Csfm; MCSF; BAP025; PG-M-CSF; Mhdabap25; BPA; PSF; HCGF; Il-3; MCGF; Csfmu; Ml; Tpo; Mgdf; Mpllg; gc; p64; [g]c; CD132; gamma(c); p460; scid; slip; DNAPK; DNPK1; HYRC1; XRCC7; dxnph; DOXNPH; DNAPDcs; DNA-PKcs |
| Application | Promote differentiation of human myeloid cells；Irradiation is not required to avoid damage to mice caused by irradiation |
Human CD34+ HSCs (3E4) were intravenously (temporal vein) engrafted into wild-type B-NDG mice and homozygous B-NDG MGMT3 mice (both sex, 24-72 hr after birth, n=15). B-NDG mice were treated with 1.0 Gy-irradiation. B-NDG MGMT3 mice were not irradiated. (A) survival rates of the mice were analyzed with Kaplan Meier survival curves. (B) Body weight. Results showed that the survival rate of B-NDG MGMT3 mice was similar to that of B-NDG mice until 18 weeks after human CD34+ HSCs engraftment and then decreased to 42.85% at 24 weeks post engraftment. But the body weight of B-NDG MGMT3 mice was significantly higher than that of B-NDG mice and increased steadily during the whole reconstitution. Values are expressed as mean ± SEM. HSCs: hematopoietic stem cells.
Human CD34+ HSCs (3E4) were intravenously (temporal vein) engrafted into wild-type B-NDG mice and homozygous B-NDG MGMT3 mice (both sex, 24-72 hr after birth, n=15). B-NDG mice were treated with 1.0 Gy-irradiation. B-NDG MGMT3 mice were not irradiated. Peripheral blood lymphocytes from the two mice after engraftment with human CD34+ HSCs were analyzed with flow cytometry. Results showed that the proportion of CD45+ cells in B-NDG MGMT3 mice reached 25% starting from 12 weeks after engraftment and continued to rise, significantly higher than that in B-NDG mice. The proportions of monocytes, MDSCs, DCs and Tregs in B-NDG MGMT3 mice were higher than that in B-NDG mice. Values are expressed as mean ± SEM.
Human CD34+ HSCs (3E4) were intravenously (temporal vein) engrafted into wild-type B-NDG mice and homozygous B-NDG MGMT3 mice (both sex, 24-72 hr after birth, n=15). B-NDG mice were treated with 1.0 Gy-irradiation. B-NDG MGMT3 mice were not irradiated. Peripheral blood lymphocytes from the two mice after engraftment with human CD34+ HSCs were analyzed with flow cytometry. Results showed that the cell numbers of all the cells analyzed from 12 weeks after engraftment in B-NDG MGMT3 mice were higher than that in B-NDG mice. Values are expressed as mean ± SEM.
