B-mSiglec15 MC38

• 311529

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B-mSiglec15 MC38

Product nameB-mSiglec15 MC38
Catalog number311529
Strain backgroundC57BL/6
NCBI gene ID620235 (Mouse)
AliasesCd33l; Cd33l3; EG620235; SIGLEC-I; Cd33l; Cd33l3; EG620235; SIGLEC-I
TissueColon
DiseaseColon carcinoma
SpeciesMouse
ApplicationB-mSiglec15 MC38 cells have the capability to establish tumors in vivo
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  • Description
  • Targeting strategy
  • Phenotypic analysis
  • Tumorigenicity

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      Description

      The mouse Siglec15 gene was insert into mouse ROSA26 site. Mouse Siglec15 is highly expressed on the surface of B-mSiglec15 MC38 cells.

      Targeting strategy

      Gene targeting strategy for B-mSiglec15 MC38.The mouse Siglec15 CDS was insert into mouse ROSA26 site in B-mSiglec15 MC38.

      Protein expression analysis

      The Siglec15 expression analysis in B-mSiglec15 MC38 cells by flow cytometry. Single cells were collected from wild-type MC38 and B-mSiglec15 MC38 cells, and analyzed by flow cytometry with anti-human and mouse SIGLEC15 antibody(SIGLEC15-5G12, in house). Mouse Siglec15 was exclusively detectable in B-mSiglec15 MC38 cells but not wild-type MC38 cells. The 2-C12 clone of B-mSiglec15 MC38 cells was used for in vivo experiments.

      Protein expression analysis of tumor cells

      B-mSiglec15 MC38 cells were transplanted into C57BL/6N mice (n=5), and on 28 days post inoculation, certain volumes of tumors as was taken to detect the expression level of the cells. Cells were analyzed by flow cytometry with anti-human and mouse SIGLEC15 antibody (SIGLEC15-5G12, in house). As shown, mouse Siglec15 was highly expressed on the surface of B-mSiglec15 MC38 tumor cells. B-mSiglec15 MC38 can be used for in vivo efficacy validation.

      Tumor growth curve & Body weight changes
      Subcutaneous homograft tumor growth of B-mSiglec15 MC38 cells. B-mSiglec15 MC38 cells (5x105) and wild-type MC38 cells (5x105 ) were subcutaneously implanted into the C57BL/6N mice (female, 5-8 week-old, n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-mSiglec15 MC38 were able to establish tumor in vivo and can be used for efficacy studies.
      Tumor growth curve of individual mice

      B-mSiglec15 MC38 tumor cells growth of individual mice. B-mSiglec15 MC38 cells (5x105) and wild-type MC38 cells (5x105 ) were subcutaneously implanted into the C57BL/6N mice (female, 5-8 week-old, n=5). As shown in panel, B-mSiglec15 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.