B-hCCN4 mice

C57BL/6-Ccn4tm1(CCN4)Bcgen/Bcgen • 111237

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B-hCCN4 mice

Catalog Number: 111237
Strain Name: C57BL/6-Ccn4tm1(CCN4)Bcgen/Bcgen
Strain Background: C57BL/6
NCBI gene ID: 8840 (Human)
Aliases: WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
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B-hCCN4 mice

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  • Phenotypic analysis

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      mRNA expression analysis

      Strain specific analysis of CCN4 gene expression in wild-type C57BL/6 mice and B-hCCN4 mice by RT-PCR. Mouse Ccn4 mRNA was detectable in ovary of wild-type C57BL/6 mice (+/+). Human CCN4 mRNA was detectable only in homozygous B-hCCN4 mice (H/H) but not in wild-type mice.

      Protein expression analysis

      Strain specific CCN4 expression analysis in homozygous B-hCCN4 mice by Western blot. Kidney and spleen tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCCN4 mice (H/H), and analyzed by western blot with anti-CCN4 antibody. CCN4 was detectable in wild type mice and homozygous B-hCCN4 mice, as the antibody is crossly reactive with CCN4 in human and mice.

      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hCCN4 mice] (Cat# 111237) was purchased from Biocytogen.