C57BL/6-Cd28tm1(CD28)Bcgen Ms4a1tm2(MS4A1)Bcgen/Bcgen • 121253
Targeting strategyGene targeting strategy for B-hCD28/hCD20 mice.
The exons 2-3 of mouse Cd28 gene that encode the extracellular domain were replaced by human CD28 exons 2-3 in B-hCD28/hCD20 mice. The exons 2-7 of mouse Cd20 gene that encode the full-length protein were replaced by human CD20 exons 2-7 in B-hCD28/hCD20 mice.
Protein expression analysis
Strain specific CD28 expression analysis in homozygous B-hCD28/hCD20 mice by flow cytometry.
Splenocytes were collected from WT and homozygous B-hCD28/hCD20 (H/H;H/H) mice, and analyzed by flow cytometry with species-specific anti-CD28 antibody. Mouse CD28 was detectable in WT mice. Human CD28 was exclusively detectable in homozygous B-hCD28/hCD20 but not in WT mice.
Strain specific CD20 expression analysis in homozygous B-hCD28/hCD20 mice by flow cytometry.
Splenocytes were collected from WT and homozygous B-hCD28/hCD20 (H/H;H/H) mice, and analyzed by flow cytometry with species-specific anti-CD20 antibody. Mouse CD20 was detectable in WT mice. Human CD20 was exclusively detectable in homozygous B-hCD28/hCD20 but not in WT mice.
