C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)BcgenTnfrsf17tm1(TNFRSF17)Bcgen Gprc5dtm1(GPRC5D)Bcgen/Bcgen • 113701
Product name | B-hCD3EDG/hBCMA/hGPRC5D mice |
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Catalog number | 113701 |
Strain name | C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)BcgenTnfrsf17tm1(TNFRSF17)Bcgen Gprc5dtm1(GPRC5D)Bcgen/Bcgen |
Strain background | C57BL/6 |
NCBI gene ID | 916,915,917,608,55507 (Human) |
Aliases | IMD18, T3E, TCRE; CD3-DELTA, IMD19, T3D; CD3-GAMMA, IMD17, T3G; BCM, BCMA, CD269, TNFRSF13A; |
CD3E expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hBCMA/hGPRC5D mice by flow cytometry. Spleen T cells were collected from wild-type C57BL/6 mice (+/+) (male, 7-week-old, n=1) and homozygous B-hCD3EDG/hBCMA/hGPRC5D mice (H/H; H/H; H/H; H/H; H/H) (male, 7-week-old, n=1). Protein expression was analyzed with anti-human CD3E antibody (BD Horizon™, 562426) and anti-mouse CD3E antibody (Biolegend, 100312) by flow cytometry. Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hBCMA/hGPRC5D mice, but not in wild-type C57BL/6 mice.
CD3E expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hBCMA/hGPRC5D mice by flow cytometry. Blood T cells were collected from wild-type C57BL/6 mice (+/+) (male, 7-week-old, n=1) and homozygous B-hCD3EDG/hBCMA/hGPRC5D mice (H/H; H/H; H/H; H/H; H/H) (male, 7-week-old, n=1). Protein expression was analyzed with anti-human CD3E antibody (BD Horizon™, 562426) and anti-mouse CD3E antibody (Biolegend, 100312) by flow cytometry. Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hBCMA/hGPRC5D mice, but not in wild-type C57BL/6 mice.
Strain specific analysis of CD3E, CD3D and CD3G mRNA expression in wild-type C57BL/6 mice and B-hCD3EDG/hBCMA/hGPRC5D mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCD3EDG/hBCMA/hGPRC5D mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CD3E, CD3D and CD3G primers. Mouse Cd3e, Cd3d and Cd3g mRNA was only detectable in wild-type mice. Human CD3E, CD3D and CD3G mRNA was exclusively detectable in homozygous B-hCD3EDG/hBCMA/hGPRC5D mice but not in wild-type mice.
Strain specific analysis of BCMA mRNA expression in wild-type C57BL/6 mice and B-hCD3EDG/hBCMA/hGPRC5D mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCD3EDG/hBCMA/hGPRC5D mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human BCMA primers. Mouse BCMA mRNA was only detectable in wild-type mice. Human BCMA mRNA was exclusively detectable in homozygous B-hCD3EDG/hBCMA/hGPRC5D mice but not in wild-type mice.
Strain specific analysis of GPRC5D mRNA expression in wild-type C57BL/6 mice and B-hCD3EDG/hBCMA/hGPRC5D mice by RT-PCR. Spleen RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCD3EDG/hBCMA/hGPRC5D mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPRC5D primers. Mouse Gprc5d mRNA was only detectable in wild-type mice. Human GPRC5D mRNA was exclusively detectable in homozygous B-hCD3EDG/hBCMA/hGPRC5D mice but not in wild-type mice.
GPRC5D expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hBCMA/hGPRC5D mice by flow cytometry. Spleen plasma cells were collected from wild-type C57BL/6 mice (+/+) (male, 7-week-old, n=1) and homozygous B-hCD3EDG/hBCMA/hGPRC5D mice (H/H; H/H; H/H; H/H; H/H) (male, 7-week-old, n=1). Protein expression was analyzed with GPRC5D positive drugs (in house) by flow cytometry. Human GPRC5D was exclusively detectable in spleen of wild-type C57BL/6 mice and B-hCD3EDG/hBCMA/hGPRC5D mice.
GPRC5D expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hCD3EDG/hBCMA/hGPRC5D mice by flow cytometry. Spleen plasma cells were collected from wild-type C57BL/6 mice (+/+) (male, 7-week-old, n=1) and homozygous B-hCD3EDG/hBCMA/hGPRC5D mice (H/H; H/H; H/H; H/H; H/H) (male, 7-week-old, n=1). Protein expression was analyzed with GPRC5D positive drugs (in house) by flow cytometry. Human GPRC5D was exclusively detectable in spleen of wild-type C57BL/6 mice and B-hCD3EDG/hBCMA/hGPRC5D mice.