B-hCD3EDG/hTROP2 mice

C57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)Bcgen Tacstd2tm1(TACSTD2)Bcgen/Bcgen • 121633

B-hCD3EDG/hTROP2 mice

Product nameB-hCD3EDG/hTROP2 mice
Catalog number121633
Strain nameC57BL/6-Cd3etm1(CD3E)Bcgen Cd3dtm1(CD3D)Bcgen Cd3gtm1(CD3G)Bcgen Tacstd2tm1(TACSTD2)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID4070,916,915,917 (Human)
AliasesEGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2; IMD18, T3E, TCRE; CD3-DELTA, IMD19, T3D; CD3-GAMMA, IMD17, T3G

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  • Phenotypic analysis

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    출판물

      mRNA expression analysis

      Strain specific analysis of CD3D and CD3G gene expression in wild type (WT) mice and B-hCD3EDG/hTROP2 mice by RT-PCR.
      Mouse Cd3d and Cd3g mRNA were detectable only in splenocytes of WT mice (+/+). Chimeric CD3D and CD3G mRNA were detectable only in homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H) but not in WT mice (+/+).

      Protein expression analysis

      Strain specific CD3E expression analysis in homozygous B-hCD3EDG/hTROP2 mice by flow cytometry.
      Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H) but not in WT mice (+/+).

      Strain specific TROP2 expression analysis in homozygous B-hCD3EDG/hTROP2 mice by western blot.
      Skin tissue was collected from wild type (WT) mice (+/+) and homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H), and analyzed by western blot with anti-TROP2 antibody. Mouse TROP2 was detectable in WT mice (+/+). Human TROP2 was detectable in homozygous B-hCD3EDG/hTROP2 mice (H/H;H/H) and B-hTROP2 MC38 cell line.