Targeting strategy
Gene targeting strategy for B-hCXCR3 mice. The full coding region of mouse Cxcr3 was replaced by human CXCR3 coding region sequences in B-hCXCR3 mice.
Protein expression analysis
Strain specific CXCR3 expression analysis in homozygous B-hCXCR3 mice by FACS. Splenocytes were collected from wild type (+/+) and homozygous B-hCXCR3 mice (H/H) stimulated with anti-mCD3ε antibody in vivo, and analyzed by FACS with species-specific anti-CXCR3 antibody. Mouse CXCR3 was detectable in wild type mice. Human CXCR3 was exclusively detectable in homozygous B-hCXCR3 mice but not in wild type mice.
