Gene targeting strategy for B-hF3 mice. The exons 1-6 of mouse F3 gene that encode signal peptide, extracellular domain, transmembrane domain, cytoplasmic region are replaced by human counterparts in B-hF3 mice. The human F3 expression is driven by endogenous mouse F3 promoter, while mouse F3 gene transcription and translation will be disrupted.

Strain specific F3 expression analysis in homozygous B-hF3 mice by flow cytometry. Endotheliocytes from lung were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-F3 antibody. Mouse F3 was detectable in wild-type mice. Human F3 was exclusively detectable in homozygous B-hF3 mice but not in wild-type mice.

Strain specific F3 expression analysis in homozygous B-hF3 mice by flow cytometry. Endotheliocytes from brain were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-F3 antibody. Mouse F3 was detectable in wild-type mice. Human F3 was exclusively detectable in homozygous B-hF3 mice but not in wild-type mice.