Targeting strategy
Gene targeting strategy for B-hGDF8 mice. The exons 1-3 of mouse Gdf8 gene that encodes the full-length protein were replaced by human GDF8 exons 1-3 in B-hGDF8 mice.
Protein expression analysis
Strain specific GDF8 expression analysis in homozygous B-hGDF8 mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), and analyzed by ELISA. GDF8 proteins were both detectable in wild-type C57BL/6 and B-hGDF8 mice, as the ELISA kit cross recognized human and mouse GDF8.
mRNA expression analysis
Strain specific analysis of GDF8 mRNA expression in wild-type C57BL/6 mice and B-hGDF8 mice by RT-PCR. Skeletal muscle RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GDF8 primers. Mouse Gdf8 mRNA was detectable only in wild-type C57BL/6 mice. Human GDF8 mRNA was detectable only in homozygous B-hGDF8 mice but not in wild-type mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hGDF8 mice] (Cat# 112413) was purchased from Biocytogen.
