Targeting strategy
Gene targeting strategy for B-hGPR75 mice. The exons 2 of mouse Gpr75 gene that encodes the full-length protein was replaced by human GPR75 exons 2 in B-hGPR75 mice.
mRNA expression analysis
Strain specific analysis of GPR75 gene expression in wild-type C57BL/6 mice and B-hGPR75 mice by RT-PCR. Mouse GPR75 mRNA was detectable in brain of wild-type C57BL/6 mice, GPR75 mRNA was detectable in brain of wild-type C57BL/6 mice and homozygous B-hGPR75 mice (H/H) due to the high genetic similarity, and we sequenced and confirmed that the replaced region of homozygous B-hGPR75 mice (H/H) is the human GPR75 sequence.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hGPR75 mice] (Cat# 112305) was purchased from Biocytogen.
