IFNGR1 expression analysis in B-hIFNGR1 mice by RT-PCR and flow cytometry. (A) RT-PCR analysis of IFNGR1. The human IFNGR1 mRNA was only detectable in splenocytes of the homozygous B-hIFNGR1 mice. (B) Peritoneal derived macrophage cells were collected from WT and B-hIFNGR1 mice, and analyzed by flow cytometry with species-specific anti-IFNGR1 antibody. Human IFNGR1 was detectable in B-hIFNGR1 mice but not in WT mice.