B-hIL27RA mice

C57BL/6-Il27ratm1(IL27RA)Bcgen/Bcgenn • 110765

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B-hIL27/hIL27RA mice
B-hIL2RA mice

B-hIL27RA mice

Product nameB-hIL27RA mice
Catalog number110765
Strain nameC57BL/6-Il27ratm1(IL27RA)Bcgen/Bcgenn
Strain backgroundC57BL/6
NCBI gene ID9466 (Human)
AliasesCRL1, IL-27RA, IL27R, TCCR, WSX1, zcytor1
제품 주문

이 페이지에서

  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hIL27RA mice. The extracellular gene sequences of mouse Il27ra gene were replaced with human IL27RA counterpart gene in B-hIL27RA mice.

      mRNA expression analysis

      Strain specific analysis of IL27RA gene expression in wild-type mice and B-hIL27RA mice by RT-PCR. Mouse IL27RA mRNA was detectable in the spleen of wild-type mice (+/+). Human IL27RA mRNA was detectable only in homozygous B-hIL27RA mice but not in wild-type mice.

      Protein expression analysis
      Strain specific IL27RA expression analysis in homozygous B-hIL27RA mice by flow cytometry. CD4+ T cells were collected from wild type mice (+/+) and homozygous B-hIL27RA mice (H/H) stimulated with anti-mCD3ε and anti-mCD28 in vitro for 72h, and analyzed by flow cytometry with species-specific anti-IL27RA antibody. Mouse IL27RA was exclusively detectable in wild-type mice and human IL27RA was detectable in homozygous B-hIL27RA mice.