Measurement of C5 inSerum
C5 expression analysis in homozygous B-NDG Hc mice by ELISA. Serum were collected from C57BL/6, B-NDG mice and homozygote B-NDG Hc mice, and analyzed by ELISA with C5 ELISA kit. Mouse C5 was detectable in C57BL/6 mice and B-NDG Hc mice. but not B-NDG mice.
Measurement of total complement hemolytic activity in serum
Hemolytic capacity analysis in homozygous B-NDG Hc mice. Representative image demonstrating level of lysis of antibody-coated SRBC following incubation with diluted serum from human, C57BL6, B-NDG, or B-NDG Hc mice. Serum from human were used as positive complement control. Compared with B-NDG, the hemolytic ability of B-NDG Hc was significantly enhanced. The order of hemolytic capacity: B-NDG Hc ≥ C57BL/6 > B-NDG.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-NDG Hc mice] (Cat# 110605) was purchased from Biocytogen.
