Targeting strategy
Gene targeting strategy for B-NDG hEPCAM mice.
The exons 3-8 of mouse Epcam genes that encode the extracellular domain and part of transmembrane domain are replaced by human EPCAM exons 2-7 in B-NDG hEPCAM mice.
The genomic region of mouse Epcam gene that encodes signal peptide, part of e transmembrane domain, and cytoplasmic portion is retained. The promoter, 5'UTR, and 3'UTR region of the mouse gene are also retained. The chimeric EPCAM expression is driven by endogenous mouse Epcam promoter, while mouse Epcam gene transcription and translation will be disrupted.
Protein Expression Analysis
Strain specific EPCAM expression analysis in homozygous B-NDG hEPCAM mice by flow cytometry. Small intestine, pancreas and lung were collected from wild-type B-NDG mice (+/+) and homozygous B-NDG hEPCAM mice (H/H), and CD45 negative cells were analyzed by flow cytometry with species-specific anti-mouse EPCAM antibody and anti-human EPCAM antibody.
Immunohistochemical (IHC) analysis of EPCAM expression in B-NDG hEPCAM mice. The heart, liver, spleen, lung, kidney, stomach, small intestine, colon and skin were collected from wild-type B-NDG mice and B-NDG hEPCAM mice (male, 7-week-old), analyzed by IHC with anti-EPCAM antibody. “+” indicates that the tissue is positive, and “-” indicates that the tissue is negative.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-NDG hEPCAM mice] (Cat# 112660) was purchased from Biocytogen.
