GIPR, gastric Inhibitory polypeptide receptor, belongs to the G-protein coupled receptor family. GIPR is expressed in many tissues, including the pancreas, stomach, brain, liver, etc. This protein plays a crucial role in regulating insulin secretion, glucose, and lipid metabolism. Mutations or changes within this gene have been associated with various health conditions including obesity and diabetes (DOI: 10.1111/j.2040-1124.2010.00022.x.).

Gene targeting strategy for B-Gipr KO mice.

• The genome sequences between part of exon 3 to exon 14 in the mouse Gipr gene were depleted in B-Gipr KO mice.

mRNA expression analysis:

• Mouse Gipr mRNA was detectable only in wild-type C57BL/6JNifdc mice but not in B-Gipr KO mice

Gene targeting strategy for B-Gipr KO mice. The genome sequences between part of exon 3 to exon 14 in the mouse Gipr gene were depleted in B-Gipr KO mice.

Strain specific analysis of Gipr mRNA expression in wild-type C57BL/6JNifdc mice and B-Gipr KO mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6JNifdc mice (+/+, 6-week-old), homozygous B-Gipr KO mice (-/-, 6-week-old), and then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Gipr primers. Mouse Gipr mRNA was detectable in wild-type mice but not in B-Gipr KO mice.